Immunohistochemistry (IHC) is the experimental process whereby primary antibodies are used to detect proteins (antigens) in cells within a tissue section. The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biologyand in antibody amplification and detection, among other things. For indirect detection, generally a HRP conjugated secondary antibody against the primary antibody is used. Horseradish peroxidase (HRP) is a 44-kDa protein that catalyzes the oxidation of substrates in the presence of hydrogen peroxide, resulting in a colored product or the release of light as one product of the reaction (chemiluminescence). We offer high quality HRP secondaries that includes antibodies against diverse species and their isotypes as well as fragment and pre-adsorbed forms. Horseradish peroxidase is widely used as a label for immunoglobulins in many different immunochemistry applications, including ELISA, immunoblotting and immunohistochemistry. A pH below 7.0 will result in weak DAB intensity; a pH above 7.6 will result in a high level of background stain. Staining is … HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides. Read our guide to chromogens to help you choose ... Substrates and chromogens for IHC Related IHC guide: introduction . Thermo Scientific Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.Features of Thermo Scientific Pierce Horseradish Peroxidase: Purified formlyophilized Many different chromogens and enhancers can be used for chromogenic detection in immunohistochemistry. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. Red. Affinity-purified anti-horseradish peroxidase antibodies are available for detection of horseradish peroxidase antigen, or for signal amplification of HRP-containing reagents. Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase. Affinity-purified anti-horseradish peroxidase antibodies are available for detection of horseradish peroxidase antigen, or for signal amplification of HRP-containing reagents. The antibody-antigen binding can be visualized in different manners. Introduction to Immunohistochemistry . If it turned brown, the tissue contains endogenous peroxidase and a blocking step is needed. Anti-Horseradish Peroxidase Antibodies.

Main Page > IHC Methods . Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase. Immunohistochemistry (IHC) is a method for detecting antigens or haptens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Horseradish peroxidase is widely used as a label for immunoglobulins in many different immunochemistry applications, including ELISA, immunoblotting and immunohistochemistry. For this purpose the sections are exposed to labelled antibodies that are directed against epitopes in the target protein. Enzymes, such as Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP), are commonly used to catalyze a color-producing reaction.